Microfluidic isolation of cancer-cell-derived microvesicles from hetergeneous extracellular shed vesicle populations
نویسندگان
چکیده
منابع مشابه
Microfluidic isolation of cancer-cell-derived microvesicles from hetergeneous extracellular shed vesicle populations.
Extracellular shed vesicles, including exosomes and microvesicles, are disseminated throughout the body and represent an important conduit of cell communication. Cancer-cell-derived microvesicles have potential as a cancer biomarker as they help shape the tumor microenvironment to promote the growth of the primary tumor and prime the metastatic niche. It is likely that, in cancer cell cultures,...
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Blood and other body f luids contain cell-derived microvesicles. The presence of microvesicles in cancer patients was already noticed in the late 1970s. Since then, the prothrombotic state in cancer patients has invariably been associated with the presence of such microvesicles. More recently, a growing body of evidence supports an important contribution of microvesicles to cancer cell survival...
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Extracellular vesicles (EVs) derived from human bone marrow mesenchymal stromal cells (MSCs) promote the regeneration of kidneys in different animal models of acute kidney injury (AKI) in a manner comparable with the cells of origin. However, due to the heterogeneity observed in the EVs isolated from MSCs, it is unclear which population is responsible for the proregenerative effects. We therefo...
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Proteomic studies of circulating vesicles are hampered by difficulties in purifying vesicles from plasma and serum. Isolations are contaminated with high-abundance blood proteins that may mask genuine vesicular-associated proteins and/or simply provide misleading data. In this brief report, we explored the potential utility of a commercially available size exclusion chromatography column for ra...
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ژورنال
عنوان ژورنال: Biomedical Microdevices
سال: 2014
ISSN: 1387-2176,1572-8781
DOI: 10.1007/s10544-014-9891-z